Effect of b vitamin addition on the biosynthesis of lactic acid by Lactobacillus delbrueckii

Aim. The main objective of this work is to investigate the effect of B vitamins on the biosynthesis of lactic acid (LA) by the Lactobacillus delbrueckii UY-2/13 strain when using corn-steep liquor as the sole nitrogen source. Methods. The influence of B vitamins was assessed using a one-factor experimental design. The lactic acid concentration in the culture broth at the end of fermentation was determined using highperformance liquid chromatography, while reducing sugars were analyzed by the modified Bertrand method. Results. The study revealed that riboflavin (B2) at a concentration of 1 mg/L increased lactic acid production by approximately 6%, whereas calcium pantothenate (B5) reduced it by about 4%. Other vitamins (PP, B9, B1, B6, H) had no significant effect on LA biosynthesis. Conclusions. Vitamins B2 and B5 have a specific effect on the biosynthesis of lactic acid by L. delbrueckii UY-2/13. Other B vitamins (PP, B9, B1, B6, and H) do not significantly influence the biosynthesis of lactic acid.