SIMULTANEOUS ESTIMATION OF PREGABALIN AND METHYLCOBALAMIN BY RP-HPLC IN BULK DRUG AND COMBINED TABLET DOSAGE FORM

The aim of this work is to develop a specific, precise, accurate, linear, simple, rapid and cost effective analytical method for the estimation of Pregabalin and Mecobalamin in combined tablet dosage form by RP-HPLC method. In the present investigation initial trials were made to develop LC conditions for the separation of Pregabalin and Mecobalamin using Pottasium dihydrogen phosphate buffer (pH6.5) as aqueous mobile phase and acetonitrile, THF as organic phase. The HPLC method was developed using Zodiac column, 250 X 4.6mm, 5 ? with Mobile phase consisting Pottasium dihydrogen phosphate buffer (pH6.5): ACN :THF(75:25:150) at 1mL/min flow rate Detection was carried out at 210nm and injection volume was 20?L.Developed method was validated in terms of linearity, range, specificity, accuracy, robustness and ruggedness. The limit of Detection was found to be 0.06ppm for pregabalin and 0.13ppm for Mecobalamin respectively.LOQ was found to be 0.41ppm for Mecobalamin and 0.20ppm for Pregabalin indicating high sensitivity method. The assay of Pregabalin and Mecobalamin tablets was performed by comparing the area of standard and sample. Assay data obtained was found to be suitable and lies well within the acceptance range of 98-102%.