METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF LAMIVUDINE, NEVIRAPINE AND ZIDOVUDINE IN BULK AND TABLET FORMULATION BY HPTLC METHOD

A high performance thin layer chromatography (HPTLC) method was developed and validated for determination of three anti-retro viral drugs, Lamivudine, Nevirapine and Zidovudine in bulk and tablet formulation. Study was performed on pre-coated silica gel HPTLC plates using Ethyl acetate: Toluene: Methanol: 25%Ammonia (4: 5: 1: 0.1 v/v/v/v) as the mobile phase. A TLC scanner set at 254nm was used for direct evaluation of the chromatograms in the reflectance/ absorbance mode. Method was validated according to ICH guidelines. The correlation co-efficient of calibration curves were found to be 0.998, 0.998 and 0.998 in the concentration range of 90-540ng/spot, 150-900ng/spot and 180-1080ng/spot for Lamivudine, Nevirapine and Zidovudine, respectively. The method had an accuracy of 98.68% for Lamivudine, 98.56% for Nevirapine and 98.65% for Zidovudine. The method had the potential to determine these drugs simultaneously from dosage forms without any interference of the tablet excipients.