Strain Improvement and Characterization of Lipase Produced by Pseudomonas Sp

The improvement of microbial strains offers the greatest opportunity for the cost reduction without significant capital investment. The purpose of the present investigation is to enhance the production by subjecting the indigenous lipase producing strain Pseudomonas aeruginosa RAS-4 to strain improvement by using UV light as a physical mutagen and immobilization. Strain improvement studies showed that mutation was the best compared to immobilization technique showing 108% higher than the parent strain using UV light as a mutagenic agent. The molecular weight of lipase was determined to be 42 kDa. Localization of the lipase specific gene was carried out by plasmid curing and acridine orange proved to be the best curing agent revealing that the lipase gene is associated with the genomic DNA.