ResearchBib Share Your Research, Maximize Your Social Impacts
Sign for Notice Everyday Sign up >> Login

Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn

Journal: Baghdad Science Journal (Vol.12, No. 1)

Publication Date:

Authors : ;

Page : 119-126

Keywords : antioxidants; Hibiscus Sabdraffia Linn.;

Source : Download Find it from : Google Scholarexternal

Abstract

A new (Reversed Phase- High Performance Liquid chromatography) RP-HPLC method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of antioxidants present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on using ultrasonic bath for extracting antioxidants. Limit of detection in ?g/ml of Vitamin C, Sabdaretine, Gossypetine, Hibiscetine, Anthocyanins, Dephinidin-3-glucoside were113.8294×10-6,123.0453×10-6,70.3681×10-6,59.6730×10-6,148.1710×10-6,and125.3481×10-6 respectively. The concentration of antioxidants found in dry spacemen of calyces of Iraqi Hibiscus Sabdraffia Linn. under study: Vitamin C, Sabdaretine, Gossypetine, Hibiscetine, Anthocyanins, and Dephinidin-3-glucoside are 258.3 ?g/g, 225.51 ?g/g, 154.975 ?g/g, 111.407 ?g/g, 439.442 ?g/g, and 185.729 ?g/g respectively.

Last modified: 2015-06-20 19:16:30