Isolation And Characterization Of Some Kind Bioactive Proteins Sponge As Antibacterial AgentJournal: International Journal of Scientific & Technology Research (Vol.3, No. 2)
Publication Date: 2014-02-15
Authors : Asri Salehi; Rauf Patong; Ahyar Ahmad;
Page : 233-236
Keywords : Indeks Terms Bioactivity; sponge; bioactive proteins; antibacterial; inhibition zone;
Abstract Has conducted research on the bioactivity of antibacterial protein fractions isolated using a polar solvent of some sponge species on the island of Barang Lompo South Sulawesi. The protein concentration was determined by Lowry method showed concentrations of the four species of sponge proteins in the crude extract in a row is 4.008 mg ml 5.376 mg ml 3.292 mg ml and 1.404 mg ml of each 500 grams of fresh weight sponge BLS 01 BLU 02 BLS 03 and BLU 04. Purification of proteins with ammonium sulfate fractionation method followed by the process of dialysis gives the result that all the ammonium sulfate fraction containing bioactive proteins where the highest inhibition zone found in BLS03 species 20-40 reaching 9.85 mm and 60-80 reaching 10.2 mm against E. coli and Staphylacoccus aureus. Testing inhibition in several variations number of bioactive proteins showed that the maximum inhibitory zone activity present in a concentration of 4000 mg mL protein of BLS03 20-40 and 60-80. Pengujian daya hambat pada beberapa variasi jumlah protein bioaktif menunjukkan bahwa aktivitas zona hambat maksimum terdapat pada konsentrasi 4000 gmL protein dari BLS03 20 40 dan 60 80 . From the results of this study indicate that the bioactive proteins from sponges potential as a base for new antibacterial drugs especially against E. coli.
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