Determination Of The Curcumin Pigment In Extract Curcuma Domestica Val From South Sulawesi Indonesia By High Performance Liquid Chromatography

Abstract Indonesia is a country endowed with a variety of medicinal plants with strong potential for therapeutic application curcuma domestica val is called kunyit is one of the most popular medicinal herbs.Its important active ingredient responsible for the biological activityofcurcuma through the major activity is antyinflammatory. Curcuma has also been reported to possess antioxidant antiallergicwound healingantibacterialantifungal and anti tumor activity.The yellow colour of curcuma is mainly due to the presence of polyphenolic curcuminoids. This experiment was aimed to determine analysis compound of curcuminoid form analysis using HPLCLC-10ATShimadzucolumn C-18 VP-ODS acetonitrilacetic acidaquabides as mobile phaseflow rate 1 mlmin and detection at 425nm.Thus the analytical method using HPLC for curcumin were feasible for quantitative analysis. The curcuminoid content from Extract Curcuma domestica Val had the highest curcumin 16.1 followed metoxycurcumin 3.2 and bisdemetoxycurcumin 2.8