Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells
Journal: Journal of Pharmacopuncture (Vol.15, No. 1)Publication Date: 2012-03-31
Authors : Choi; Eun-A; Yoon; Jeung-Won; Choi; Hak-Joo; Kim; Dong-Hee; Yoo; Hwa-Seung;
Page : 7-11
Keywords : cytokine; GM-CSF; IL-1β; IL-6; lipopolysaccharide; TNF-α;
Abstract
Aim: Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Methods: RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). Results: IS4 concentration inhibited the production of interleukin-1beta (IL-), interleukin-6 (IL-6), and granulocyte-macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50 ) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF-) stimulated by LPS. Conclusion: IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.
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