Differential Expression of Gene Profiles in MRGX-treated Lung Cancer
Journal: Journal of Pharmacopuncture (Vol.16, No. 3)Publication Date: 2013-09-30
Authors : Yong-Kyun Kwon; Seung-Yeul Lee; Hwan-Soo Kang; Jung-Suk Sung; Chong-Kwan Cho; Hwa-Seung Yoo; Seungjin Shin; Jong-Soon Choi; Yeon-Weol Lee; Ik-Soon Jang;
Page : 30-38
Keywords : gene ontology; lung cancer; microarray; modified regular ginseng extract (MRGX);
Abstract
Objectives: Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles. Methods: To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network. Results: One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. Conclusions: Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.
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