Validation of Eupatorium triplinerve Vahl Leaves, a Skin Care Herb from East Kalimantan, Using a Melanin Biosynthesis Assay
Journal: Journal of Acupuncture and Meridian Studies (Vol.5, No. 2)Publication Date: 2012-04-30
Authors : Enos Tangke Arung; Harlinda Kuspradini; Irawan Wijaya Kusuma; Kuniyoshi Shimizu; et al.;
Page : 87-92
Keywords : 7-methoxycoumarin; B16 melanoma cells; Eupatorium triplinerve Vahl; melanin biosynthesis; tyrosinase;
Abstract
In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360μM (L-tyrosine was used as the substrate) and above 2840μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
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