PRODUCTION OF BIODIESEL FROM Calophyllum Inophyllum L. OIL BY LIPASE ENZYME AS BIOCATALYST

Production of biodiesel from Calophyllum inophyllum L oil through transesterification by enzyme as catalyst and methanol as solvent has been done. Determinations of molecular weight of lipase enzyme used SDS - PAGE anal ysis. While production of biodiesel through transesterification process was conducted with molar ratio of oil to methanol in 1:12 at a temperature of 30 ? 45 o C for 3 hours use 3 % of enzym concentration. Results of analysis SDS - PAGE yielded molecular we ight enzyme were26, 31, and 59 kDa . 1 H - NMR result showed theoretical biodiesel conversion was 74.84%, while the experimental result was 66.07%. GC - MS spectrums showed that biodiesel contents there are methyl palmitate (3,47%), methyl linoleate (4,57%), me thyl oleate (9,93%), and methyl stearate (2,65%