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Simultaneous Detection of Insecticides and Growth Regulators by Single Chromogenic Spray Reagents

Journal: International Journal of Chemical and Physical Sciences(IJCPS) (Vol.2, No. 1)

Publication Date:

Authors : ;

Page : 7-11

Keywords : suicidal; Homicidal; Postmortem Toxicology; Insecticides TLC; Bromophenol blue silver nitrate reagents.;

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Abstract

Insecticides are widely used in India for agriculture purpose1-2. Due to easy availability of insecticides with new trade names and mixture of different class they are knowingly or unknowingly used for homicidal and suicidal poisoning purposes. Thin layer chromatography is found to be the best option for the identification of these insecticides. Presently number of chromogenic reagents is used to identify different class of insecticides from the biological materials which is found to be tedious, laborious, time consuming and increases the analytical cost. Hence a single unique chromogenic spray reagent for detection of different commonly used insecticides like organophosphorous, carbamate, organochloro, sysnthetic pyrethroids and plant growtrh regulators by thin layer chromatoraphy technique. The present paper report if 10% of aqueous sodium hydroxide and 0.1% Bromophenol Blue in 1% silver nitrate solution in absolute alcohol at room temperature sprayed on these insecticide gives different colour spots like blue, purple, yellow, black green for different insecticides. Limit of detection of these reagents is about 10μg.

Last modified: 2013-07-25 18:40:02