Detection of Nitrobenzene in Biological Materials by Thin Layer Chromatography

A thin layer chromatographic method is developed for the detection of nitrobenzene in biological material. Nitrobenzene on reduction gives aniline, a primary amine which on diazotization and coupling with β- naphthol, gives orange coloure μ spot (i.e. aniline dye). The detection limit for nitrobenzene on TLC plate in biological material is 5μg. The biological impurities such as amino acids, fats etc. present in visceral material do not interferes in the test. The methods has been successfully employed for the detection in forensic samples.