THE ROLE OF 2, 4 ?D AND NAA IN CALLUS INDUCTION OF ACHYRANTHES ASPERA AND ITS SECONDARY METABOLITE STUDIES

In the present investigation callogenic and secondary metabolite studies of Achyranthes aspera were carried out, stems were used as explants, callus was induced by using two different plant growth hormones 2,4-D (Dichlorophenoxyacetic acid) and NAA (1-Naphthalene acetic acid) in different concentration and in combinations supplemented in the MS Medium ( Murashige and Skoog). The effect of growth hormones (2, 4-D and NAA) in callus induction was studied at the second and fourth week of culture. During the second week of growth, media supplemented with the growth hormones 2,4-D and NAA separately showed best response at a concentration of 2.0mg/L and 4.0mg/L respectively. With a combination of both hormones in the media, best response was seen at the concentration of 2.0+2.0mg/L of 2, 4-D and NAA. Similar results were observed in the fourth week of culture. Under the morphological character study of the callus induced by the hormones, A different observation was made with the color of the calli ranging from large brown to brownish yellow. The presence of steroids, alkaloids and sugars in the callus was also analyzed by performing thin layer chromatography using various spray reagents accordingly