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Starch Enzyme Hydrolysis ? Experimental and Kinetics

Journal: Electronic Journal of New Materials, Energy and Environment (Vol.1, No. 2)

Publication Date:

Authors : ; ;

Page : 32-37

Keywords : starch hydrolysis; α-amylase;

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Abstract

In order to study the condition of starch hydrolysis with thermostable ?-amylase the influence of the substrate concentration, temperature and pH were investigated. The kinetics parameters ? Km and Vmax were determined using Michaelis ? Menten equation and the other its forms ? Lineweaver - Burk, Hanes - Woolf and Eadie - Hofstee. It was found that reaction rate has maximum value at pH 5.8 and temperature of 75 ºC. The values of Km are between 60.42 ? 65.72?g.ml-1, and Vmax has values be-tween 10.89 and 11.35 ?g.ml-1.min-1.

Last modified: 2015-10-11 07:40:28