Starch Enzyme Hydrolysis ? Experimental and Kinetics
Journal: Electronic Journal of New Materials, Energy and Environment (Vol.1, No. 2)Publication Date: 2015-10-11
Authors : Ina Karadashka; Stilijan Tchaoushev;
Page : 32-37
Keywords : starch hydrolysis; α-amylase;
- Starch Enzyme Hydrolysis ? Experimental and Kinetics
- Kinetics of Hydrolysis of Cassava Starch (Manihot esculanta): Effect of Addition of K and Mg Ions on Reducing Sugar
- Use of pullulanase as a biocatalyst for starch hydrolysis: Part 1. Study of the effect of pullulanase on maize amylopectin starch
- Effects of Inhibition of Starch Branching Enzyme on in situ Degradation of Endosperm Starch During Rice Seedling Growth
- Kinetics and Mechanism of Hydrolysis of O-Hydroxy Benzaldehyde - Aniline Spectrophotometrically
Abstract
In order to study the condition of starch hydrolysis with thermostable ?-amylase the influence of the substrate concentration, temperature and pH were investigated. The kinetics parameters ? Km and Vmax were determined using Michaelis ? Menten equation and the other its forms ? Lineweaver - Burk, Hanes - Woolf and Eadie - Hofstee. It was found that reaction rate has maximum value at pH 5.8 and temperature of 75 ºC. The values of Km are between 60.42 ? 65.72?g.ml-1, and Vmax has values be-tween 10.89 and 11.35 ?g.ml-1.min-1.
Other Latest Articles
- Review on “The Counter-Revolution of Science. Studies on the Abuse of Reason” by F.A. Hayek
- Use of Coconut Fiber As Reinforcement in Bamboo Leaf Ash Blended Cement - Based Composite Panels
- Reliability - Based Determination of the Coefficients of Lateral Earth Pressure on Retaining Walls Subjected to Seismic Loading
- Theoretic and methodological bases of mechanisms of state management
- The Influence of Compactive Effort on the Desiccation - Induced Volumetric Shrinkage of Compacted Bagasse Ash Treated Foundry Sand As Hydraulic Barrier Material
Last modified: 2015-10-11 07:40:28