Comparison of Conventional and PCR based detection of Plasmodium falciparum and Plasmodium vivax infections in human blood
Journal: Journal of Advances in Biology (Vol.3, No. 3)Publication Date: 2011-12-17
Authors : Afshan Iqbal; Rabia Mushtaq; Ferhana Hussain; Mahjabeen Saleem; Mamoona Naz; Beenish Kashif Hashmi;
Page : 248-252
Keywords : Malarial infection; Plasmodium falciparum; Plasmodium vivax; Nested PCR;
Abstract
Blood samples from the 100 malarial parasite (MP) negative suspected individuals and 50 malarial patients were collected in EDTA vacutainers. Genomic DNA from each individual was amplified using nested PCR assay for 18S smaller ribosomal subunit gene to detect malarial parasites in low grade parasitemia. Data was statistically analyzed afterwards. MP test diagnosed only 1% malarial infection in collected blood samples whereas it increased up to 4% using molecular methods. MP test was unable to detect mixed infection whereas the nested PCR assay detected 3% P. falciparum and 4% P. vivax mixed infections in blood samples. Sensitivity (P value) of nested PCR was statistically 0.00 which showed that PCR technique is highly significant for detection. From the present data it is concluded that PCR is more sensitive and specific technique to be used as a diagnostic tool for malarial parasite as compared to the traditional microscopical analysis as practiced in all hospitals. It should be applied in biochemical labs (pathological labs) of hospitals so that it helps in reduction of false negativity of malarial diagnosis.
Other Latest Articles
- Correlation of serum Nitric Oxide and hs-CRP in non-smoker and non-diabetic patients with Coronary Artery Disease
- Searching of Standard Comet Assay Parameters for Detecting Lymphocyte DNA Damages Using Fourteen Different Test Conditions
- Evaluating porcine red blood cells in acute African swine fever virus (genotype II) infection in peripheral blood
- Picroside II could protect the cerebral ischemic injury by reducing the content of free radical and enhancing the activity of antioxidase in rats
- Structural DNA Nanotechnology: From Design to Applications
Last modified: 2015-10-12 14:47:09