RETICULOCYTE ASSESSMENT AS A BIOMARKER FOR BONE MARROW ERYTHROPOIETIC FUNCTION IN PREGNANT WOMEN ATTENDING ANTENATAL CLINIC IN BENIN CITY, NIGERIA

A decrease in iron stores in pregnancy is accompanied by a significant rise in the reticulocyte count .This study was aimed at using reticulocyte production index as a biomarker for erythropoietic function in pregnant women. Packed cell volume was determined using sysmex® Automated Hematology System. Reticulocyte count was determined using the supra vital staining techniques. It was observed that there was a significant decrease in packed cell volume at stage 4 when compared to stage 1, 2 or 3, (P < 0.05) and significant increase in reticulocyte count , reticulocyte index and reticulocyte production index at stage 4 when compared to stage 1, 2 or 3 (P < 0.05). The significant decrease in packed cell volume coupled with an increase in Reticulocyte production index is a reflection of an increased erythropoiesis activity of the bone marrow, occasioned by the increased fetal demand for micro-nutrients which is associated with pregnancy. Proper compliance to vitamin B12, folic acid and iron supplementations are advocated during pregnancy.