Isolation Stem Cells From Human Dental Pulps And Differentiation Them To Adiposite And Osteoblast

Objective: in this study we tried to isolated stem cells from human primary dental pulp. These cells are capable of differentiating into a variety of cell types including neural cells, adipocytes, and odontoblasts, osteoblast Background & Aim: Among different source for stem cells, less attention has been paid to the human dental pulp. Human dental pulps are the best and new source for stem cells, and have potential to different to all kinds of cells. So the aim of this study was to evaluate the pulp ability for produce stem cells. Materials and Methods: Normal exfoliated human deciduous incisors collected from6- to 12-years-old children. The pulp was separated from the crown and digested with trypsin .stem cells cultured in DMEM(Dulbecco’s Modified Eagle’s Medium), supplemented with 20% fetal bovine serum in a 37°C incubator with 5% co2. The cells were differentiated into the osteoblastic and adipocytic lineages in different subcultures. Results: Stem cells are able to growth and proliferate unlimitedly and differentiate to all kinds of the cells in human body. Their life time is very long and they remain their potential for long periods of time. For this reasons today many physicians and researchers consider them as a therapeutic agent to cure diseases. One of the most important advantaged of dental pulp stem cells, is that isolated from primary dental, that are thrown away usually. Various scaffolds have been used to cell culture. Stem cells were observed to grow in colony forming units, mainly composed of a fibroblast-like cell population. Conclusion: This study show that pulp contains cells with high proliferation capacity. The cells can be used for clinical application. Cells can be considered as an easy accessible source of mesenchymal stem cells for stem cell research and tissue engineering.