Generation of hepatocyte from mouse mesenchymal stem cells in Vitro
Journal: Journal of Advances in Biology (Vol.7, No. 3)Publication Date: 2014-12-18
Authors : zahraa kamel zedan; Shahlaa M. Salih; Shalal M. Hussain;
Page : 1353-1359
Keywords : Mesenchymal stem cells; Hepatocytes; Hepatocytes growth factors;
Abstract
This study was designed to generate hepatocyte cells from mesenchymal stem cells (MSCs) isolated from mouse bone marrow via using culturing protocols for this goal. Cells of MSCs were isolated by collecting the thigh bone of 50 albino mice, both femur and tibia were collected and cells were flushed from bones and MSCs have been isolated based on the ability of MSCs to adhere on plastic surfaces. MSCs reactivity to CD45, CD90, CD34 and CD105 were tested by immunocytochemistry. Isolated MSCs exhibited positive reactivity towars CD105 and CD90 cell surface markers. MSCs were negative for the hematopoietic surface markers including CD34 and CD45. Differentiation into hepatocyte was induced by adding hepatogenic specific growth factors (Hepatocytes growth factors (HGF), Fibroblast growth factor4 (FGF4), oncostatin M and dexamethasone) to the differentiation medium. Cells were examined after differentiation protocol and characterized using immunocytochemistry analysis for Albumin and alpha fetoprotein. Positive results were observed indicating the potential ability of the isolated bone marrow MSCs to differentiate into Hepatocytes.
Other Latest Articles
- Interaction between Toxoplasmosis and Some Biochemical Parameters in Aborted Women in Al-Kut City
- Surveillance ecological study of cellular responsesin three marine edible bivalve species to Cd present in their marine habitat,Mediterranean sea in Alexandria, Egypt
- Effects of Urea and Imazethapyr on Orobanche Crenata germination, Radicle extension, Incidence and Faba Bean Growth and Yield
- The effect of ZJXG decoction on the serum levels of GH, CT, PTH and VitD3 in femur fracture rats
- Effect of Ground Seeds of Picralima nitida Incorporated Diets on Serum Lipid Profile of Albino Rats
Last modified: 2015-10-19 19:00:52