Mercury Removal From Petroleum Based Industries Wastewater By P. Putida

ABSTRACT Mercury pollution is one of a primary environmental issue and public health problem. The purpose of this research is to remove the mercury using pure culture Pseudomonas putida ATCC 49128 at optimum growth parameters such as technique of culture acclimatization time and speed of incubator speed. In this study the optimum growth parameters ofP. putida were obtained to achieve the maximum of mercury removal. Thus a field study were carried out at two different location based on petroleum industrial plants in Peninsular Malaysia. Processes involved in this research which is P. putida behavior in rehydrating free-dried growing method growth parameters and optimum operating conditions. Analysis that carried out are turbidity total dissolved solid and suspended solid where related to growth of P. putida. Efficiency of mercury removal from actual petroleum based industries plant 1 P1 wastewater with the presence of 1000 ppb Hg increased from 84 after 4 hours to 90.5 after 96 hours. For plant 2 the sample with presence P. putida and nutrient broth had the highest of mercury removal which is 97.27. This results show by using P. putida is efficient for mercury removal from actual petroleum.