Study On Callus Initiation And Plantlet Regeneration Ability Of Some Rice Genotypes

Abstract An experiment was conducted with mature embryos of three HYD and resistant rice varieties BRRIdhan-29 BR-14 BINA dhan-8 as explants to investigate the appropriate growth condition for callus induction as well as plant regeneration on MS basal medium supplemented with different concentration of auxin and cytokinin. Different concentrations of 2 4-D 0.0 1.5 2 2.5 3 mgL-1 were used for callus induction. Among the concentrations 2.0mgL-1 2 4-D was best concentrations for callus induction 85 callus size 4.533mm and callus weight 81.847mg in 10.66days and the highest maintaining ability was found in BR-14 at 2.0mgL-1 2 4-D 88 in callus size 5.143a and callus weight 87.50mg in 6.50days. Different concentrations of kinetin 0.0 0.4 0.6 0.8 10 mgL-1 with a constant concentration of NAA 0.5mgL-1 were used for plant regeneration from callus. Again BRRI dhan-29 on T3 MS 8 mgL- Kn 0.5 mgL- NAA was found to be best in shoot length 6.00cm with highest shoots initiation 97.5 and the highest .number of shoots per callus 5.25 on T2 MS 6 mgL- Kn 0.5 mgL- NAA in 22.25days. In combination BRRI dhan-29 the highest number of roots per explant 7.25 root length 2.31cm was found in T3 MS 0.6 mgL-1 IBA and the highest root initiation 97.50 was on T2 MS 0.5 mgL-1 IBA.