Rapid And Sensitive High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method For Determination Of Danshensu In Rat Plasma
Journal: Journal of Proteomics and Genomics Research (Vol.1, No. 3)Publication Date: 2014-01-30
Authors : Jin-Qian Wang; Yan Ting; Yin-Fang Chena; Yue-Sheng Wang;
Page : 43-49
Keywords : High-performance liquid chromatography/tandem mass spectrometry; danshensu; Protein precipitation; Rat plasma;
Abstract
A sensitive, specific and rapid high-performance liquid chromatography/tandem mass spectrometry (hplc? ms/ms) method has been developed and validated for the determination of danshensu (dss) in rat plasma in the present study. The analytes were separated on a c18 column (50 mm×2.1 mm, 1.7 μm) and a triplequadrupole mass spectrometry equipped with electrospray ionization (esi) source was applied for detection. The simple protein precipitation was applied to extract dss from the plasma (about 80%). The method was linear over the concentration ranges of 50?1000 ng/ml for dss. The lower limit of quantitation (lloq) of dss was 50 ng/ml. The intra-day and inter-day relative standard deviation (rsd) were less than 15% and the relative error (re) were all within 15%. Finally, the method was successfully applied to support the pharmacokinetic study after guanxinsu solution was orally administrated to the sprague?dawley rat, respectively.
Other Latest Articles
- CONTRACTION AND DEVELOPMENT OF SCOLIOSIS
- THE MODERN ASPECTS OF MALIGNANT TUMORS RADIODIAGNOSIS OF THYROID BODY
- BREAST CANCER METASTASES IN CHOROID
- Determination Of The Proteomic Response To Lapatinib Treatment Using A Comprehensive And Reproducible Ion-Current-Based Proteomics Strategy
- FORECASTING THE INCIDENCE OF HODGKIN’S LYMPHOMA USING NEURAL NETWORKS
Last modified: 2015-11-16 20:27:17