Simultaneous Spectrophotometric Estimation of Nystatin and Triamcinolone

The simple, accurate, precise and economic spectrophotometric method in UV/VIS region has been developed for the determination of Nystatin and Triamcinolone Acetonide in Topical formulation. Nystatin and Triamcnolone Acetonide are widely aimed for treatment of fungal infection. The wavelengths selected for Simultaneous equation method were 304 nm and 240 nm i.e. the respective λmax of both the drugs. This method was applied to the assay of the drug in marketed formulation, which were found in the range of 97 - 98 % of the labeled value for both Nystatin and Triamcinolone acetonide. The method was validated for linearity, accuracy and precision. Hence, the method herein described can be successfully applied in quality control of combined pharmaceutical dosage form.