IN VITRO CALLUSING AND EFFECT OF GROWTH REGULATORS ON IN VITRO PROPAGATED WITHANIA (CULTIVATED & WILD) THROUGH COTYLEDONARY LEAFS

Leafs as well as Cotyledonary excised small explants of Ashwagandha were responsible and introduced to evaluate the effect of various growth regulators upon the in vitro micropropagation processes. Explants were applied to generate callus, shoot and root regeneration. In the first experiment small segments of leaf were cultured on MS basal medium added with mixture of 2,4 ? Dichlorophenoxyaceticacid (2,4?D), naphthalene acetic acid (NAA) and indole butyric acid (IBA). The agile pharmacological components of Withania somnifera components are withanolides a Steroidal lactones ring with ergostane skeleton framework and alkaloids (Elsakka et al 1990; Gavande et al 2015). The active contents or substances of Indian Withania somnifera or Ashwagandha are normally withaferin-A and withanolide-D, both are generally present in leaves, stems and roots of the plant in high quantity, are used as a source of drugs. This procedure was standardized and stablished for very easy large scale mass propagation of ashwagandha medicinal plant. Callus formation was seen best in Murashige and Skoog media supplemented with NAA (1.0-3.0mg/L) after 16-20 days.