The Synthesis and Characterization of New Cross-Linkable Quinolone and Quinoline-Based Luminescent Lanthanide Chelates

Luminescent lanthanide probes possess long emission lifetime, which enables their hypersensitive detection in time-resolved mode that avoids short-lived background fluorescence. Sharply spiked emission spectra of lanthanide probes and large Stokes shifts further enhance detection sensitivity, which is about 1000 fold higher than that for regular fluorescent probes. The wide spread of this promising technology is limited by high cost of commercially available compounds, which is mainly due to their complex structure and laborious synthetic procedure. In this study, new efficient strategies to simplify the synthesis of the probes were explored. New lanthanide chelates, containing click-, and amine-reactive cross-linking groups, which are highly bright and can be produced with high yield, are synthesized and characterized.