COMPARATIVE STUDY OF THE EFFECT OF ELECTROMAGNETIC FIELDS, INTERFERON - BETA AND TRANSFER FACTOR IN THE RECOVERY OF CHRONIC ULCERS IN TREATED RATS

Objectives: To construct a device capable of emitting electromagnetic fields (EMF) of ultralow frequency, based on Helmholtz coils and determine whether the frequency of total lymphocytes CD4+, CD8+ is modified in Wistar rats with chronic ulcer. Metodology : 1) A tri - axial prototype was designed to generate EM F to a frequency of 120 Hz. 2) Biological tests, 7 groups of Wistar rats, with previous ulcer formation; a control group with application of sterile water for injection (ip). Experimental groups: 1) Application of EMF (120 Hz) every 24 hours for 12 days; 2 ) Interferon - ? and 3) transfer factor, with doses (ip) every 24 hours for 12 days. Peripheral blood samples were taken to measure frequency total of lymphocytes, and biopsies of ulcers. Results: The frequency of total lymphocytes increased significantly (P <0.05) as well as frequency of CD4 + and CD8 +; EMF, INF - ? and TF a trend to normal ranges significantly (P <0.05), compared with the negative control group was observed. Ulcers of the negative control rats began scar tissue within 96 hours, whereas with FT and EMF start time of scar tissue was 72 hours and with the INF - ? - ? , cicatrization started in 120 hours.