Spectrophotometric determination of Eflornithine hydrochloride through schiff’s base system using pdab reagent in pharmaceutical preparation

A rapid, economic and highly sensitive spectrophotometric method was developedf or the quantification of eflornithine hydrochloride (DFMO). The method involves the reaction DFMO with para-dimethylamino benzaldehyde (PDAB) in acidic medium (pH 5.4) which results in an orange-coloured product exhibiting maximum absorbance λmax at 563. The proposed method can be utilized as a stability indicating assay. The different experimental parameters affecting the derivatization reaction were carefully studied and incorporated into the procedure. Under the described conditions the apparent molar absorption coefficient (έ563) is 4.9 × 103 L/mol cm. The linear equation is Y= 1.60 +0.038X (μg/mL) in the range of 5?40 μg/mL of DFMO with a correlation coefficient r = 0.9992, and the detection limit (LOD) is 11.842 μg/mL. The average recovery of the target compound is 100.07% with a limit of quantification (LOQ) of 39.47 μg/mL. The mechanism of the derivatization reaction is proposed and advantages of the proposed method are discussed. The method was validated in terms of accuracy and precision and was successfully applied to the determination of DFMO in its pharmaceutical dosage form. The proposed method is useful for routine analysis of DFMO in quality control laboratories.