In silico?enzyme function prediction in?hypothetical proteins of Mycobacterium bovis AF2122/97

Background: Mycobacterium bovis linked to cause bovine tuberculosis has been investigated and functionally annotated for several hypothetical proteins’ ability of enzyme activity as per the conserved domain present. Methods: As M. bovis genome codes in total 25% hypothetical proteins, we used CDD BLAST, INTERPRSCAN, PFAM and CATH domain search programs to find the enzyme functionality in these proteins. Results and discussion: Programs reported 347 hypothetical proteins posses enzymatic activity as per the enzyme domain conserved in the sequences at variable confidence levels. Conclusion: These proteins could be the markers, to investigate about their fate in the metabolism of M. bovis.