Genetic variation in the narrow-clawed crayfish (Astacus leptodactylus) populations as assessed by PCR-RFLP of mitochondrial COI gene
Journal: Molecular Biology Research Communications (Vol.4, No. 4)Publication Date: 2015-12-01
Authors : Majidreza Khoshkholgh; Sajad Nazari;
Page : 225-237
Keywords : Astacus leptodactylus; Mitochondrial DNA; PCR-RFLP; Genetic diversity;
Abstract
The genetic variation and population structure of narrow-clawed crayfish (Astacus leptodactylus) was examined by means of polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) analysis of the cytochrome oxidase subunit I (COI) of mitochondrial DNA. A total of 194 adult specimens were collected from seven sample sites including, two in the south Caspian Sea and one each in Anzali wetland and Aras reservoir and three rivers Chafrood, Masule Rudkhan and Siah Darvishan. The PCR products were digested with 19 restriction enzymes and five enzymes revealed polymorphism patterns (DdeІ, MboІ, TaqI, RsaІ and HinfІ). Twenty eight composite haplotypes were showed with the number of haplotypes in each population sample ranging from 8 to 13. Private haplotypes were found at very low frequencies. Two regional (Siah Darvishan River and Astara) groups were clearly recognized by cluster and molecular variance model (AMOVA) analyses (P<0.0001). Each of these groups revealed dominant haplotypes while these haplotypes play less important rule in population structures of the other geographic areas. Intrapopulation haplotype (h) and nucleotide (π) diversities were high for each locality, ranging h=0.7560±0.030 and π= 0.00334±0.00301, respectively. Results of this study discerned two genetically divergent populations of narrow-clawed crayfish including Siah Darvishan River and Astara. Thus, the population structure of the narrow-clawed crayfish, as inferred from mtDNA analysis, is constituted by genetically separate groups that nearly reflect their geographic distribution.
Other Latest Articles
- Promoter hypermethylation of KLOTHO; an anti-senescence related gene in colorectal cancer patients of Kashmir valley
- Description of a myxozoan parasite Myxobolus sp.n PKB2014 from an edible fish, with emphasis on its molecular characterization
- Molecular systematics and distribution review of the endemic cyprinid species, Persian chub, Acanthobrama persidis (Coad, 1981) in Southern Iran (Teleostei: Cyprinidae)
- Expression of growth hormone gene during early development of Siberian sturgeon (Acipenser baerii)
- The binding assessment with human serum albumin of novel six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands
Last modified: 2015-12-13 19:21:28