In vitro antioxidant, anti-inflammatory and anticholinesterase activities of Rumex vesicarius L

Objective: To investigate phenolic content, flavonoid content, the antioxidant, anti-inflammatory, and anticholinesterase activities of ethyl acetate extracts of Rumex vesicarius L from leaf, stem, flower and seed. Materials and Methods: The assessment of the antioxidant potential of crude leaf, stem, flower and seed extracts, using the ferric reducing antioxidant power (FRAP) assays and 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and â-Carotene radical scavenging capacity assays was carried out. Furthermore, the 15-lipoxygenase inhibitory activity was evaluated by the ferrous oxidation- xylenol orange (FOX) assay method. Moreover, Cholinesterase inhibitory activities were examined using Ellman’s colorimetric method. The total phenolic content measured by Folin-ciocalteu method was as well conducted. Results: The raw seed, flower, stem and leaf extracts of Rumex vesicarius L was found to contain a high content of total phenolic content (36.44 to 54.15 mg GAE/g DW) and flavonoids (12.36 to 21.97 mg CE/g DW). All extracts organs exhibited a higher antioxidant activity and inhibitory effect of radicals scavenging activity against FRAP, ABTS and â-Carotene. For the anti-inflammatory activity, the extracts tested had significant 15-lipoxygenase inhibitory ranging from 33.82 to 62.45% and had a noticeable inhibition towards AChE and BChE. Conclusion: the results suggest that the seed, flower, stem and leaf of Rumex vesicarius L exhibited high antioxidant and anti-inflammatory, this property may be related with high quantity of phenolic and flavonoid in extracts, this plant can be considered as a good source of natural antioxidant and anti-inflammation drugs as well as potent anticholinesterase effect.