Anticarcinogenic effects of cinnamomum verum on HL60 leukemia cell lines

Background: Phyto medicine are focused more by recent researchers for the treatment of different types of chronic diseases including cancer, because of its radical scavenging effect, antioxidant capacity and anticarcinogenic potentiality. The present study was aimed to assess to the presence of bioactive compounds and its anticarcinogenic activity of Cinnamomum verum (C.verum) bark. Methods : The quantitative and qualitative phytochemical analysis was carried out with the ethanol and methanol extracts C.verum. GCMS analysis, of methanolic extract was done. The radical scavenging activity and the antioxidant potentials were assessed by thio barbituric assay, metal chelating activity, superoxide anion radical scavenging activity, phospho molybdenum assay. Further studies were progressed with in vitro cell cytotoxicity assay, DNA fragmentation assay and cell morphology assay with ethanolic extract of C.verum using HL 60 leukemia cell lines. Results and Discussion : The quantitative phytochemical analysis showed the presence of tannin, saponin, alkaloids, flavonoids, polyphenols and steroids. The total phenolic and flavanoid contents were estimated as 176.7mg/g and 153.2mg/g. From GCMS results the bioactive phytochemicals and their structural characterizations were identified. The radical scavenging activity of ethanol and methanol extracts of C.verum showed better scavenging activity against free radical generation. In in vitro antioxidant assay the results showed both the extracts ethanol and methanol of C.verum have significant antioxidant potentiality. The results of cell cytotoxicity assay show good inhibitory effect. DNA fragmentation analysis confirms the characteristic nuclear fragmentation in the late stage of apoptosis. The morphological identification of DNA shows cellular shrinkage, membrane blebbing, and typical nuclear fragmentation. Conclusion : The present study proves that C.verum bark is a good source of bioactive compounds and exhibit the anticarcinogenic effect through various mechanism.