IN ? VITRO EVALUATION OF ANTIBACTERIAL ACTIVITY OF SILYBUM MARIANUM SEEDS OIL, ETHANOLIC EXTRACT AND ANTIBIOTICS AGAINST ISOLATED BACTERIA

The present study aimed to investigate the antibacterial activity of ethanolic extract of Silybum marianum seeds, , it s oil, Chloromphincol, Rifampin and Methicillin against isolated bacteria, evaluation is done in-vitro by using hole plate method for appearance zone of inhibition. Antimicrobial susceptibility test showed that their were no antibacterial activity of siylmarin extract while the oil showed very mild effect against Enterbacter ludwigii, Klebsiella pneumonia and Pontoea gaviniae. Chloromphincol showedeffects aginst most the isolated bacteria except Citrobacter freundii, Enterobacter cloacae and Psudomonous aerogenosa, Rifampin affect only on Pontoea gaviniae while Methicillin didn’t show any affects .Chloromphenicol showed intermediate effect on Enterbacter ludwigii, Enterobacter sakazakii, Klebsiella pneumonia, Pantoea agglomeruns, Proteus mirabilis, Salmonella typhomurium,, while Staphylococcus hominins were Susceptible, most the isolated bacteria were resistance to Rifambin except Pontoea gaviniae gave intermediate inhibition zone. in case of Rifampin no effect were recorded.