Tobacco ,oxidative stress and protein carbonyl content

Aim - The highest risks of exposure of tobacco for both adults and adolescents, the major concern is that tobacco consumption is associated with certain other behaviors, long range consequences and show characteristic facial changes. Definite role for reactive oxygen species (ROS) in the tissue destruction that characterizes in tobacco addicts and protein carbonyl is the most widely used biomarker for oxidative damage to proteins, and reflects cellular damage induced by multiple forms of ROS. The purpose of this study is to determine malondialdehyde and protein carbonyl content in tobacco addicts with Otorhinological manifestations and to find an association between them. Material and Methods : - Total 300 subjects were selected. 150 were healthy control group and 150 patients were presented with one or more otorhinolaryngological manifestations. In addition to demographic data, information regarding tobacco use was documented in the subjects. Results : - Both benign and malignant conditions of ENT were common in male patients. MDA, marker of oxidative stress and protein carbonyl content were increased in both benign and malignant conditions of otorhinolaryngological. Both values were higher in malignant lesions than benign lesions. There was significant positive correlation between MDA and protein carbonyl levels in cancer patients. Conclusion : - Increase in the oxidative stress (MDA) to such an extent that damages the DNA and increase in the protein carbonyl content would leads to cancerous condition. Protein Carbonyl estimation can be done in the unknown primary lesions and dysplastic lesions.