Detection of Genetic variation in tissue culture clones of date palm using ISSR markersJournal: International Journal for Scientific Research and Development | IJSRD (Vol.3, No. 10)
Publication Date: 2016-01-01
Authors : Rukam; Parakhia M. V; Rathod V. M; Rathod; P. J; Padhiyar S. M;
Page : 37-40
Keywords : Date palm; clones; ISSR;
Date palm is a plant having high nutritional value and long life (yielding up to 100 years). Phoenix dactylifera requires 2-5 males for pollination of 100 femalesÃ¢â?¬â?¢ plant depending up on genetic and environment factors. Therefore paternity variation expected to very low according to PCR based techniques, Even though we have tried to find out genetic variation among tissue culture cloned plant. Tissue culture technique can be used for genetic improvement of date palm. The main purpose of this study was to evaluate the genetic variation in the tissue culture clones of date palm by using ISSR primers among mother and itÃ¢â?¬â?¢s two clones. The plant DNA was extracted and subjected to detection of genetic variation in two groups of date palm using ISSR primers. In this study ISSR primers produced monomorphic bands within group-1 and group-2. Genetic variation in tissue culture clones of date palm was not detecte by UBC primer series.
Other Latest Articles
Last modified: 2016-01-06 18:52:29