ALTERED OXIDATIVE METABOLISM IN SALIVARY GLANDS OF RATS DURING APPLICATION OF METHACRYLIC ACID METHYL ESTER ONTO ORAL MUCOSA

The experiment carried out on 20 Wistar male rats weighing 180 ? 220 g was designed to study the properties of nitroxidergic system and related changes in free radical processes in the tissues of submandibular salivary glands (SG) in rats as well as their protein-synthesizing function for a 30-day application of methacrylate acid methyl ester onto the oral mucosa, which is used as a monomer for the manufacturing of removable dentures. It has been shown that under the experimental conditions in the SG tissues there is a significant increase in total activity of NO-synthase (NOS) and in the concentration of stable products of nitric oxide oxidation such as nitrite ions, while SG protein-synthesizing function reduces (decrease in the activity of ornithine decarboxylase and α-amylase). At the same time the production of superoxide anion radical by NADPH-dependent (microsomal and NOS) and NADH-dependent (mitochondrial) electron transport chains in SG tissues increases while the process of lipid peroxidation under significantly reduced antioxidant capacity and activity of superoxide dismutase and catalase is activated.