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STRUCTURAL CHANGES IN RAT TESTES EXPOSED TO BACTERIAL LIPOPOLYSACCHARIDE E. COLI ON THE 3RD DAY AFTER EXPOSURE

Journal: Journal of the Grodno State Medical University (Vol.51, No. 3)

Publication Date:

Authors : ; ; ;

Page : 23-27

Keywords : lipopolysaccharide; testis; spermatogenesis;

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Abstract

The aim of the study was to evaluate structural changes in the testes of rats on the third day after exposure to the bacterial lipopolysaccharide gram-negative microorganism Escherichia coli. Bacterial lipopolysaccharide Escherichia coli (serotype 0111: B4, manufactured by Sigma, USA) was administered to male rats at a dose of 50 mkg/kg intraperitoneally one time. Research methods included histological, morphometrical, electron microscopy. In the experimental animals there were detected a decrease in the number of interstitial endocrine cells by 13.85% and a decrease in the area of their nuclei by 23.06%; a decrease in the number of sustentocytes by 25.65% and reduction of the area of their nuclei by 17.15%; a decrease in the number of spermatocytes by 26.54%. Electron microscopic examination revealed swollen basement membrane of the tubules and ultrastructural changes in sustentocytes and spermatocytes. Single administration of E. coli bacterial lipopolysaccharide to male rats at a dose of 50 mg/kg i.p., causes on the 3rd day after exposure development of pathological changes in the testes of the animals, which results in a reduction of interstitial endocrinocytes; reduction in the amount of sustentocytes and spermatocytes while reducing the size of their nuclei; changes in the ultrastructure of spermatogenic epithelium in the form of large numbers of phagolysosomes in the cytoplasm of sustentocytes and reduction of cristae in mitochondria.

Last modified: 2016-01-28 19:24:07