Aktivitas enzim lipase alkali dari bakteri dalam surfaktan
Journal: Majalah kulit, karet, dan plastik (Vol.14, No. 26)Publication Date: 1999-12-30
Authors : V. Sri Pertiwi Rumiyati; Retno Indrati;
Page : 84-94
Keywords : ;
Abstract
Activity and stability of alkaline lipase from bacteria (strain AS, KB and SP) were studied in containing of surfactants at 0,05% and 0,10%. The survactanswere used in research, these were consisted of four anionic surfactants (cetyl trimethyl ammonium bromide, cetyl pyridium chloride, cetyl dimethyl ammonium bromide) and there nonionic surfactants (Triton X ? 100, tergitol, and nonidet P.40). Production of enzyme was produced in initial medium pH 7,5; incubation at 37oC for 48 h. These research showed that activity of alkaline lipase from strain A S-1; AS-2; KB-4; SP-2 and SP-13 was stable in containing of anionic surfactants and nonionic surfactants at 0,05% and alkaline lipase from KB-8 and SP-1 were showed decrease of activity (relatif activity < 80 %). Strain KB ? 4 was prodused alkaline lipase which stable in containing of anionic surfactants, cationic surfactants and nonionic surfactants at 0,05% & 0,10%. It was had high activity (activity relatif 90-125%).
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