In Vitro Seed Germination and Plantlets Development of Canthium Coromandelicum (Burm. f.) Alston

A protocol for in vitro seed germination and plantlets development of Canthium coromandelicum (Burm. f.) Alston a medicinal plant belonging to family Rubiaceae was developed which is used in the treatment of scabies, ringworm, cough, indigestion, snake bite. It shows various pharmacological properties like antioxidant, anti-inflammatory, antirhuematic, wound healing and diuretic activity. High frequency in vitro clonal propagation protocol was standardized from the nodal explants derived from in vitro raised 30 days old seedlings. Knudson’s C (KnC) medium was the best suitable medium for seed germination and seedling development. Maximum shoot multiplication frequency was obtained on Murashige & Skoog medium supplemented with 4 mg/l 6-benzyl amino purine (BAP) alone. The elongated shoots were subcultured for successful rooting on ? MS with 3 mg/l NAA. The in vitro raised plantlets were acclimatized in green house and progressively transplanted to natural conditions with 70% survival.