AN INVESTIGATION ON THE CYTOTOXICITY AND APOPTOTIC EFFECT OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES ON MCF-7 AND A549 CELL LINES USING WEED SETARIA VERTICILLATA L

Novel utilization of weed synthesized nanoparticles plays major role in treatment against many cancer cells and pathogenic microbes. Green synthesis is more economical, ecofriendly and large scale production of nanoparticles. This study investigates on cytotoxic and apoptotic effect of setaria verticillata synthesized silver nanoparticles against Breast cancer cell (MCF-7) and Lung cancer cell (A549) lines. The synthesized silver nanoparticles were characterized by High resolution-transmission electron microscopy (HR-TEM). The optical property of silver nanoparticles was determined by selected area electron diffraction (SAED). The invitro cytotoxicity and apoptotic effect of synthesized silver nanoparticles was evaluated against Breast cancer cells (MCF-7) and lung cancer cells (A549) by MTT assay, Trypan blue dye assay and DNA fragmentation assay. The size of silver nanoparticles was spherical with average size of 12±4 nm. Electron diffraction pattern shows the silver nanoparticles were crystalline in nature with Face centered cubic (FCC) structure. The MTT assay shows the % CTC50 is 897.05?g/ml for MCF-7 and 402.75 ?g/ml for A549 cell lines respectively. Trypan blue dye assay indicates cytotoxic effects increased with increase in concentration of silver nanoparticles upto 1000 ?g/ml. The DNA fragmentation assay of silver nanoparticles treated MCF-7 and A549 shows the double strand breaks shows formation of DNA ladders. The invitro cytotoxic effect of setaria verticillata synthesized silver nanoparticles was toxic against Breast cancer cell (MCF-7) and lung cancer cell (A549). Keywords: Silver nanoparticles, MCF-7, A549, HR- TEM, MTT assay.