A study on Rh incompatibility and frequency of weak D among blood donors and patients at a tertiary care referral teaching hospital in Tirupati, Andhra Pradesh
Journal: Journal of Clinical and Scientific Research (Vol.4, No. 4)Publication Date: 2015-10-01
Authors : Deepthi Krishna G; Sreedhar Babu KV; Arun R; Jothibai DS;
Page : 281-284
Keywords : Rho D antigen; Coombs test;
Abstract
Background: Rh D antigen is the next most important after ABO antigens in the field of transfusion medicine. Weak D refers to reduced expression of D antigen on the red blood cell that requires an extended testing with indirect antiglobulin test (IAT) to get detected. Clinical importance of weak D arises when labelling the donor and patient, as the donor is labelled as D positive, patient as D negative. Methods: In our center all blood donor and patient samples are tested for ABO and Rh D by conventional tube technique using two anti-D reagents; anti-D immunoglobulin M (IgM) monoclonal and a blend of anti-D IgM and immunoglobulin G (IgG). The blood samples which were negative for agglutination by immediate spin method were further tested for weak-D using IgG anti-D in the IAT phase with low ionic strength solution (LISS)/Coombs’gel card. Results: A total of 46,654 blood samples were tested (22,326 donors and 24,328 patients) during the period January 2012 to August 2014. Among these 43,771 (93.82%) were Rh D positive and remaining 2,883 (6.18%) were Rh-D negative. A total of 30 individuals (16 donors and 14 patients) were weak D positive constituting 1.04% of Rh-D negatives and 0.06% of total individuals screened. Conclusions: This study shows the prevalence of weak D antigen in our population who are representative of Rayalaseema region of Andhra Pradesh. It also stresses the need to identify individuals with variant D (rather than weak or partial D) and to inform them about their status as donor and recipient of blood/organ.
Other Latest Articles
- Vitamin D supplementation therapy ? comparison of efficacy of three different protocols
- Burden of hepatitis C virus infection and its genotypes among the blood donors at Tirupati, Andhra Pradesh
- Urinary enzymes and microalbuminuria as biomarkers of renal dysfunction after contrast administration
- Rising incidence of acute kidney injury ? the emerging significance of “pill” burden
- Various Security Issues and their Remedies in Cloud Computing
Last modified: 2016-02-20 15:30:49