Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG)

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinical signs demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulcer on the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown in chorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virus in cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasal swab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reaction and cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs. Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done three times. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm, rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processed for hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosin staining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cell of membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodies against BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be used as a medium for detection of IBR.