BIOFILM FORMS IN MONO- AND MIXED STAPHYLOCOCCI SPECIES CULTURE IN COMBINATION WITH LACTOBACILLI
Journal: Біологічні Студії Studia Biologica (Vol.9, No. 34)Publication Date: 2015-12-02
Authors : G. Lavryk; O. Kornijchuk;
Page : 89-98
Keywords : biofilm; Lactobacillus; Staphylococcus aureus; Staphylococcus epi? der?mіdis.;
Abstract
An unsufficient effectiveness of antimicrobial treatment of many infectious inflammation processes is often associated with the formation of the microbial Biofilms forms. The ability to a biofilm formation was investigated in clinical S. aureus and S.epidermidis strains isolated from patients with acne vulgaris and reference L. рlantarum 8P-A3 strain. For evaluation of the film formation, the spectrophotometric measuring of the optical density of structures at the bottom of plastic Petri dishes was used. Item condition of the film structures was also controlled by using microscopic method. It was established that the optical densities of monospecific biofilms formed by S. aureus and S. epidermidis were 1.69±0.77 and 0.60±1.50 OD, respectively, and the planktonic forms of the reference strain was 0.09±0.06 OD. Biofilm structure of L. plantarum 8P-A3 has optical density of 1.79±1.07 OD. Fluorescent microscopy allowed to estimate the proportion of the viable cells in the biofilm. The viable cells were absent after 48 h of mixed-species formation of L. рlantarum 8P-A3 and S. aureus biofilm. Local application of the preparations from lactobacilli contributes to the effectiveness of treatment of the inflammation caused by staphylococcus biofilm forms
Other Latest Articles
- EFFECT OF 1,4-NAPHTHOQUINONE AMIDE DERIVATIVES ON MORPHOLOGICAL CHANGES IN LOACH EMBRYOS AND LARVAE
- FREE RADICALS PROCESS IN BIRD’S LUNG UNDER THE ACTION OF SODIUM HYPOCHLORITE
- Biochemical and haematological changes in peripheral blood of rats exposed to chlorpyrifos: protective effect of vitamins A and E combination
- THE DISTURBANCE OF OXIDANT-ANTIOXIDANT BALANCE IN RAT COLONIC MUCOSA AFTER ANTIBIOTIC THERAPY
- T-type CALCIUM CHANNEL AND Kir 4.1 POTASSIUM CHANNEL GENE EXPRESSION IN THALAMIC RETICULAR NUCLEUS AND SOMATOSENSORY CORTEX OF WAG/Rij RAT STRAIN
Last modified: 2016-03-02 03:39:57