SEMAX INFLUENCE ON RAT CARDIOMYOCITE APOPTOTIC CELL DEATH IN IRREVERSIBLE ISCHEMIA AND ISCHEMIA-REPERFUSION

5 groups of male rats were investigated: control group; irreversible ischemia with drug and without; the animals with reperfusion after 2.5 hours of ischemia with drug and without it. Samples for light microscopy (haematoxylin-eosin and TUNEL-analysis) of the risk area (anterior wall of left ventricle) and conditionally intact myocardium (lateral wall and interventricular septum) were taken 72 hours after intervention. Semax were injected intraperitoneally at a dose of 150 mg / kg after 15 minutes and after 2 hours 15 minutes after ligation of coronary artery. The necrosis was formed in the anterior wall area both under irreversible ischemia and ischemiareperfusion. Substantial morphological changes also developed in areas of relatively intact myocardium. These signs are accompanied by a significant increase of apoptotic index. Semax had no effects on the morphology of myocardium, but significantly reduced apoptosis of cardiomyocytes.