QUANTIFICATION OF PIPERINE BY HPTLC METHOD

In present study HPTLC method has been carried out for determination of piperine of different manufactures. These days’ numbers of Pharmaceutical companies are manufacturing Tribhuvankirti Rasa. In this study five samples out of which one is lab prepared (self-prepared) Tribhuvankirti Rasa and four are market preparations. The alcoholic extract of Tribhuvankirti Rasa and standard piperine samples were applied on TLC aluminium plate pre coated with silica gel 60 F 254 and developed using toluene: Ethyl acetate (7: 3) v/v as a mobile phase, using UV detector at wavelength of 254 nm. Content of marker compound in the samples were found similar. Key Words: Tribhuvankirti Rasa, Piperine, HPTLC