MOLECULAR CHARACTERIZATION OF OIL CONTENT OF SOYBEAN

Soybean is an important leguminous crop having 40-44% protein & 20% oil. In soybean, presence of different type of fatty acid in different quantity is major problem associated with genetic constitution of crop species. The aim of present research work was to review content of different types of fatty acid in cultivars of soybean at primary level. Thirteen cultivars release by M. K. V., Parbhani were studied. Fourteen soybean specific SSR primers were used to reveal genetic diversity of soybean cultivars. Thirteen cultivar of soybean have different percentage of oil, MAUS-1 genotype of soybean is having highest percentage of oil (22.34%) followed by JS-93-05 (21.44%). Fourteen primers had shown diversity in a range of 54.5% to 83.3% having different banding pattern. From nutrition point of view, low linolenic, low palmatic & high oleic acid contain is desired in soybean oil. The primary objective of this study is to analyze different binding pattern of different fatty acid specific primer at molecular level. Overall study revealed that SSR marker can reveal the quantitative trait loci (QTL) governing the trait oil content of soybean. the diversity revealed by molecular characterization of soybean had shown that different cultivar had different fatty acid content. From the results of this study, a further study can be undertaken to correlate polymorphism of this data with quantitative estimation of different fatty acids (linolenic, palmatic, oleic). MAUS-1 has highest oil content but found some major linolenic acid specific band missing in the DNA profile. From the nutrition point of view low linolenic acid content is desired in genotypes of soybean. MAUS-1 can be the target germplasm of further study on quantitative estimation of linolenic acid due to its height oil content.