“EFFECT OF SMA-DMSO GNP ON CELL SURVIVAL AND DNA DAMAGE ANALYSIS IN MCF-7 & HEK CELL LINE BY MTT AND COMET ASSAY”

Male hormonal and non-hormonal contraceptives have been subject of great interest at present at global level. SMA-DMSO complex is one such non-hormonal male contraceptive compound which has been extensively studied by many researchers and found promising results. Use of such SMA-DMSO GNP Compound has been found to contain some adverse cyto-toxic and carcinogenic effect as well. However some research reports have also reported the decreased toxic effect of SMA-DMSO after encapsulating the compound SMA-DMSO GNP. Present study aims to study the effect of different dosage of SMA-DMSO GNP on cell survival and DNA damage. MCF 7 and HEK cell viability and DNA damage has been tested against SMA-DMSO GNP in the dosage range of 25 nM to 200 nM using MTT and COMET assay at different time period of 24 hr to 48 hr. The result shows that SMA-DMSO GNP significantly increases the survival of cell line MCF 7 & HEK cell lines. Along with DNA damage was also found negligible in different time period by comet assay test. The following study may conclude that SMA-DMSO GNP have better chance of application as contraceptive solution to men’s population. However further interrogative assays need to be carried to assess its genotoxic potential, allergenicity & carcinogenicity.