Microbial profile of neonatal intensive care unit isolates and changes in the pattern of antibiotic sensitivity

Background: Neonatal Sepsis is most frequent cause for admission in Neonatal Intensive Care Unit (NICU). This is a major cause of neonatal mortality and morbidity worldwide. Aim and objectives: To study microbial profile of isolates from sample of NICU, to determine the susceptibility pattern of commonly encountered pathogens. Materials and methods: Retrospective evaluation of Laboratory data of samples from NICU of Dhiraj General Hospital for microbial profile and antibiotic sensitivity patterns from June 2014 to September 2015 was done. Standard procedures for isolation, identification and antibiotic sensitivity testing were followed. Results: Total 141 samples were tested, out of which 37 were culture positive. A total of 41 isolates were obtained which included Klebsiella - 10, Acinetobacter - 7, Pseudomonas - 5, E. Coli - 4, S. Aureus - 4, CONS - 6, Enterococcus - 2, and Candida ? 3. Among Gram negative organisms, most frequently encountered organisms were Klebsiella and Acinetobacter. Conclusion: In present study, frequently encountered organisms were Klebsiella and Acinetobacter which showed sensitivity to mainly Imipenem. Sensitivity to other routinely used antibiotic was variable. Changing sensitivity patterns should be monitored continuously and guidelines should be revived. Early identification of organism and appropriate antibiotic usage minimizes mortality and morbidity.