EFFECT OF DIFFERENT DILUTION MATERIALS USAGE ON INDONESIAN PERANAKAN ONGOLE BULL SPERM QUALITY DURING COOLING PROCESS

The research aimed to examine the effect of dilution based on tris and egg yolk with CEP-2 on motility, viability and sperm membrane integrity of Peranakan Ongole bull during cooling process. The randomized block design with 10 replications from 10 ejaculations of Peranakan Ongole bull was applied in this study. Treatments applied were T0 = Tris aminomethane + 20% egg yolk, T1 = CEP-2 + 10% egg yolk, T2 = CEP- 2 (Non BSA) + 10% egg yolk, T3 = CEP-2 (Non BSA) + 15% soya bean extract and T4 = CEP-2 + 15% soya bean extract. Observations on individual sperm motility, viability and sperm membrane integrity were directly carried out after dilution each day up to the tenth day and after dilution and kept cooled. The study showed that dilution based on tris aminomethane + egg yolk could maintain the semen quality included sperm motility, viability and cattle sperm membrane integrity during cooling process. Spermatozoa of Peranakan Ongole bull diluted with tris aminomethane + 20% egg yolk at the seventh day of storage had a progressive motility (45.75%), viability (49.25%) and membrane integrity (42.70%). Keywords: Tris aminomethane, egg yolk, motility, membrane integrity, viability.