Effect of UV-B (280-320 nm) radiation in IN-VITRO culture from callus explant of black gram cultivars

The in vitro regeneration was carried out in black gram (Vigna mungo) under UV-B irradiation for the first time. Callus induction was tried with leaf explants harvested from control and UV-B irradiated three cultivars of Vigna mungo (Vamban 1, Vamban 2 and Vamban 3) to study their viability for Callus suspension cells. UV-B delayed callus induction and depressed biomass accumulation. However, the suspension cells were 100% viable in all the cultivars of Vigna mungo under the control conditions. The cultivar Vamban 2 (V2) is the more sensitive one to the enhanced UV-B radiation treatment. The suspension cells which received 10 and 15 min of enhanced UV-B radiation only 53% and 38% cells were viable, respectively.