PREPARATION OF PARATUBERCULOSIS VACCINE FOR ANATOLIAN WILD SHEEP: PRELIMINARY RAPORT
Journal: International journal of ecosystems and ecology science (IJEES) (Vol.1, No. 3)Publication Date: 2011-10-01
Authors : Osman ERGANİŞ H. Hüseyin HADİMLİ Kürşat KAV Zafer SAYIN Aslı BALEVİ Yasemin PINARKARA M. Seyyide TEMİMHAN;
Page : 55-64
Keywords : Wild Sheep; humoral immunity; paratuberculosis infection;
Abstract
Four different vaccine (inactivated bacterin+aliminium hydroxide (Al(OH)3), inactivated bacterin+mineral oil, live+mineral oil, gamma irridation+mineral oil) from Mycobacterium avium subs. paratuberculosis (MAP) strain isolated from Anatolian Wild Sheep (Ovis gmelinii anatolica), Etlik MVKAE from bovine strain (one) and subunit (one) vaccine were prepared. Commercial GudairTM vaccine were used as positive control. Each vaccine were subcutaneously administared to 15 Akkaraman lambs 2 months age old. Ten lambs were unvaccinated as negative control. Lambs to 5 in each vaccine groups were divided for seropotens. After vaccination at 40 days, lambs in groups of vaccine ( lambs to 10, 7 groups, 70 lambs) and control (10 lambs) were orally challenged with MAP isolate into milk at 2x109 cfu/ml. Taking into account seropotens groups, antibody titres were monitored by ELISA. According to the first five post-vaccination serologic measurements; Gudair vaccine provided faster antibody production but developed 4 vaccine formulation (inactivated bacterin+mineral oil, gamma irridation + mineral oil, live+mineral oil, live Etlik) were determined stimulated to humoral immunity as measured in the positive control at 5th measurements. In addition, INF-?, skin test by PPD johnin, lenfosit transformation test and clinical efficiences were observed. It has shown that data in laboratuar efficiency tests of vaccine prepared from Anatolian Wild Sheep isolate in domestic lambs could also be protective against paratuberculosis infection for Anatolian Wild Sheep.
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